Following are required for Genome Browser:
•Build information for the organism of your experiment – Build is information of the chromosomes present in the organism and their lengths
•Chromosome number, start and end position for the probes/genes in the technology of your experiment. - This information is required to show data in Genome Browser.

Yes, genes in an experiment can be mapped to chromosomal location in Genome Browser of GeneSpring. The mapping is based on chromosome number, start and end locations.