Home > Q15. Why does GeneSpring add the variance stabilization value 16 to the expression values for exon arrays, why not for expression arrays?
For Exon arrays, the background subtraction is done on a pool of probes having similar GC content (which is not the case with expression arrays). This typically results in probe sets having small expression values leading to an unreliable estimate of the variance. To offset this, an adhoc value of 16 is added to the expression values of all the probe sets.