Home > Q16. Why only variance stabilization value 16 is added to the expression values for exon arrays, why not some other value?
The reason for adding 16 is that it is generally considered a low enough number that it will provide the required stabilization effect without changing suppressing true signal values. 8 and 32 are other options that are commonly used. usually values smaller than 16 are due to noise and you could have values 8 and 16 causing a fold change of 2, purely due to noise.