Home > Q17. I found that even after using baseline to median of all samples, the result of statistical analysis is same, although the profile plot is different with different baseline transformation. I want to know the reason for this situation?
Statistical analysis results would not change based on the Baseline transformation to median of all samples as the actual deviation between the conditions for the particular entity would not change and therefore there would not be any change in the P-values across all the experiments based on the baseline transformation. Baseline transformation provides the user better visualization when comparing the relation between two groups without affecting the downstream analysis.